Marreese Speights and Hilton Armstrong will man the frontcourt for the Warriors, whose frontcourt is extremely short-handed without O'Neal, David Lee (rest), Draymond Green (rest) and Andrew Bogut (ribs).
Nene will play on Wednesday but he has "insisted" that the Wizards keep him under 20 minutes.
He recently returned from a sprained MCL which he tweaked in his first game back, and Washington will proceed with due caution. He would sit out entirely if there were nothing at stake, but a win combined with a Nets loss would shift Washington up a seed in the playoff standings.
Draymond Green is expected to sit out Wednesday's game as a precaution.
Rusty Simmons on Twitter
Green is coming off a massive game on Monday and with nothing at stake on Wednesday the Warriors will give him some rest. His health and stamina will be crucial for their playoff hopes, since Andrew Bogut's absence with a broken rib means we'll see plenty of small frontcourts with Green alongside David Lee.